[35] Assay and purification of phospholipase A2 from human synovial fluid in rheumatoid arthritis
RM Kramer, RB Pepinsky - Methods in enzymology, 1991 - Elsevier
Publisher Summary In rheumatoid arthritis, the synovial fluid contains phospholipase A 2
(PLA 2) in a soluble form and in relatively high amounts. Several procedures have been
developed for purifying PLA 2 from rheumatoid synovial fluid, but low yields are reported,
with typical recoveries accounting for only 4–8% of the total enzyme activity. This chapter
describes a purification protocol that exploits the remarkable stability and solubility of this
PLA 2 at low pH and results in yields of total enzymatic activity of greater than 50%. With this …
(PLA 2) in a soluble form and in relatively high amounts. Several procedures have been
developed for purifying PLA 2 from rheumatoid synovial fluid, but low yields are reported,
with typical recoveries accounting for only 4–8% of the total enzyme activity. This chapter
describes a purification protocol that exploits the remarkable stability and solubility of this
PLA 2 at low pH and results in yields of total enzymatic activity of greater than 50%. With this …