Collection of untargeted metabolomic data for mammalian urine applying HILIC and reversed phase ultra performance liquid chromatography methods coupled to a Q …
Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most
frequently applied analytical platform in the untargeted metabolomic study of mammalian
urine. Here we describe two complementary UPLC-MS methods for metabolomic analysis or
urine, a reversed phase C 18 method and a hydrophilic interaction liquid chromatography
(HILIC) method. We discuss the inclusion of pooled quality control (QC) samples and a
recommended analysis list construction. Up to 96 injections can be performed every 24 h …
frequently applied analytical platform in the untargeted metabolomic study of mammalian
urine. Here we describe two complementary UPLC-MS methods for metabolomic analysis or
urine, a reversed phase C 18 method and a hydrophilic interaction liquid chromatography
(HILIC) method. We discuss the inclusion of pooled quality control (QC) samples and a
recommended analysis list construction. Up to 96 injections can be performed every 24 h …
Abstract
Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most frequently applied analytical platform in the untargeted metabolomic study of mammalian urine. Here we describe two complementary UPLC-MS methods for metabolomic analysis or urine, a reversed phase C18 method and a hydrophilic interaction liquid chromatography (HILIC) method. We discuss the inclusion of pooled quality control (QC) samples and a recommended analysis list construction. Up to 96 injections can be performed every 24 h, and up to 2000 metabolites can be routinely detected.
Springer