A fully-automated, six-plex single molecule immunoassay for measuring cytokines in blood
We report a system and assay for performing fully-automated measurement of 6 proteins
simultaneously with single molecule sensitivity. The system combines handling of samples,
reagents, and consumables, with a module for imaging single molecule arrays (Simoa) to
enable immunoassays that have high sensitivity (~ fg/mL), are multiplexed, and are fully-
automated. A 6-plex cytokine Simoa assay for IL-6, TNF-α, GM-CSF, IL-10, IL-1β, and IL-1α
was developed on the system. The assays had limits of detection in the range 0.01–0.03 …
simultaneously with single molecule sensitivity. The system combines handling of samples,
reagents, and consumables, with a module for imaging single molecule arrays (Simoa) to
enable immunoassays that have high sensitivity (~ fg/mL), are multiplexed, and are fully-
automated. A 6-plex cytokine Simoa assay for IL-6, TNF-α, GM-CSF, IL-10, IL-1β, and IL-1α
was developed on the system. The assays had limits of detection in the range 0.01–0.03 …