T-cell activation is enhanced by targeting IL-10 cytokine production in toll-like receptor-stimulated macrophages
RM Walk, ST Elliott, FC Blanco, JA Snyder… - ImmunoTargets and …, 2012 - Taylor & Francis
Toll-like receptor (TLR) agonists represent potentially useful cancer vaccine adjuvants in
their ability to stimulate antigen-presenting cells (APCs) and subsequently amplify the
cytotoxic T-cell response. The purpose of this study was to characterize APC responses to
TLR activation and to determine the subsequent effect on lymphocyte activation. We
exposed murine primary bone marrow-derived macrophages to increasing concentrations of
agonists to TLRs 2, 3, 4, and 9. This resulted in a dose-dependent increase in production of …
their ability to stimulate antigen-presenting cells (APCs) and subsequently amplify the
cytotoxic T-cell response. The purpose of this study was to characterize APC responses to
TLR activation and to determine the subsequent effect on lymphocyte activation. We
exposed murine primary bone marrow-derived macrophages to increasing concentrations of
agonists to TLRs 2, 3, 4, and 9. This resulted in a dose-dependent increase in production of …
T-cell activation is enhanced by targeting IL-10 in toll-like receptor stimulated macrophages
RMM Walk, FC Blanco, JA Snyder, S Elliott… - Journal of the …, 2011 - journals.lww.com
Methods IL-10 siRNA constructs were designed and tested for their ability to suppress IL-10
secretion from a macrophage cell line by quantitative real-time polymerase chain reaction.
Murine primary bone marrow derived macrophages were harvested, lipofected with IL-10
siRNA, and stimulated with CpG oligonucleotide, a TLR-9 agonist. Macrophage tumor
necrosis factor-alpha (TNF-alpha) and IL-10 secretions were measured. Autologous murine
splenocytes were then exposed to both supernatants from the stimulated macrophages and …
secretion from a macrophage cell line by quantitative real-time polymerase chain reaction.
Murine primary bone marrow derived macrophages were harvested, lipofected with IL-10
siRNA, and stimulated with CpG oligonucleotide, a TLR-9 agonist. Macrophage tumor
necrosis factor-alpha (TNF-alpha) and IL-10 secretions were measured. Autologous murine
splenocytes were then exposed to both supernatants from the stimulated macrophages and …