[HTML][HTML] Protein targeting to exosomes/microvesicles by plasma membrane anchors
Animal cells secrete small vesicles, otherwise known as exosomes and microvesicles
(EMVs). A short, N-terminal acylation tag can target a highly oligomeric cytoplasmic protein,
TyA, into secreted vesicles (Fang, Y., Wu, N., Gan, X., Yan, W., Morell, JC, and Gould, SJ
(2007) PLoS Biol. 5, 1267–1283). However, it is not clear whether this is true for other
membrane anchors or other highly oligomeric, cytoplasmic proteins. We show here that a
variety of plasma membrane anchors can target TyA-GFP to sites of vesicle budding and into …
(EMVs). A short, N-terminal acylation tag can target a highly oligomeric cytoplasmic protein,
TyA, into secreted vesicles (Fang, Y., Wu, N., Gan, X., Yan, W., Morell, JC, and Gould, SJ
(2007) PLoS Biol. 5, 1267–1283). However, it is not clear whether this is true for other
membrane anchors or other highly oligomeric, cytoplasmic proteins. We show here that a
variety of plasma membrane anchors can target TyA-GFP to sites of vesicle budding and into …